Kinetics of specific humoral immune response of mice infected with low doses of Trichinella spiralis, T. britovi, and T. pseudospiralis larvae

Infectivity, reproductive capacity and distribution of Trichinella spiralis and T. pseudospiralis larvae in experimentally infected sheep.

Twelve Merino sheep were experimentally shown to be susceptible to infection with Trichinella spiralis or T. pseudospiralis by feeding on infected carcasses of mice or by oral intubation with recovered muscle larvae. The larvae recovered from the sheep showed variable tissue distribution. The diaphragm and tongue were most affected. The viability of the recovered larvae was confirmed by success...

Cross resistance against challenge infection in mice infected with Trichinella spiralis or T. pseudospiralis.

Infectivity, persistence and serological response of nine Trichinella genotypes in rats.

Domestic and sylvatic Trichinella genotypes were evaluated for infectivity, muscle larvae persistence, and host antibody responses in rats. Groups of rats were inoculated with T. spiralis, T. nativa, T. britovi, three genotypes of T. pseudospiralis (from USSR, USA, and Australia), T. murrelli, Trichinella T6, and T. nelsoni, respectively. The muscle larvae intensity (larvae per gram), total lar...

Expression of MyoD and myogenin in muscles of mice experimentally infected with Trichinella spiralis or Trichinella pseudospiralis.

We developed a detection system for myogenic regulatory factors such as MyoD and myogenin. Adapting the method we performed a longitudinal analysis of such regulatory factors after infection with T. spiralis and T. pseudospiralis. MyoD and myogenin were expressed from the early phase of cystogenesis in T. spiralis infection. The expression returned to the normal level after 18 days from the inf...

Evaluation of two PCR-based techniques for molecular epidemiology in Finland, a high-endemic area with four sympatric Trichinella species.

Trichinella larvae collected from wildlife, domestic and synanthropic animals in Finland were identified to species by two molecular techniques: Random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) and the recently described multiplex PCR. The RAPD-PCR was very sensitive to the sub-optimal preservation muscle larvae and resulting in weak and smeared bands on the gels for such...